Sure Test HIV Cassette 1s, also known as SURE CHECK® HIV 1/2 Assay, is a single-use, disposable rapid test kit for qualitative detection of antibodies to HIV-1 and HIV-2 in human specimens, serving as an aid in diagnosing HIV infection. This immunochromatographic assay features a self-contained device with a sample well, test lines (T1 for HIV-1, T2 for HIV-2), and control line (C), using colloidal gold-conjugated HIV antigens for visual interpretation—no instruments needed. It requires just 5μl of fingerstick whole blood (preferred for self-testing), venous blood, serum, or plasma; add sample and buffer, wait 15 minutes for results: two lines indicate reactive (positive), one control line negative, or invalid if no control. FDA-approved, CLIA-waived for professional use, and WHO-prequalified for self-testing, it's 99.9% specific and sensitive, suitable for adults and high-risk screening. Store unopened at 2-30°C with <60% humidity, away from sunlight; use immediately once opened, discard biohazardously. While user-friendly with pictorial instructions, positives must be confirmed via lab tests (e.g., Western blot); negatives don't rule out acute infection. Not for neonates or as sole diagnostic—consult healthcare providers for counseling and follow-up.